ACQUITY UPLC SYSTEM
Waters ACQUITY UPLC® System offers drmatic improvements
in speed, sensitivity and resolution. Whether you work in methods development or quality assurance, in drug discovery and development or testing for safety in food supplies, you seek a productivity edge. With the ACQUITY UPLC® System, now you can get more information in a single, short run than you’ve ever seen with HPLC.
UltraPerformance LC® (UPLC®) technology starts with unique sub-2 µm small-particle chemistries. Chromatographers no longer need to choose between speed and resolution—with UPLC you get both.
Instrument Designed for Chemistry; Chemistry Designed for the Instrument
The ACQUITY UPLC System has been holistically designed to match the performance needs of our innovative column chemistries with robust hardware, easy-to-use software and
specialized support services.
1. Small, pressure-tolerant particles; 2. High-pressure fluidic modules; 3. Minimized system volume; 4. Negligible carryover; 5. Reduced cycle times; 6. Fast response detectors; 7. Integrated system software and diagnostics.
With UPLC you have the ability to work more efficiently with higher speed, sensitivity and resolution at a much wider range of linear velocities, flow rates and backpressures to obtain superior results. The ACQUITY UPLC System’s effective system delay volume is < 120 µL, independent of operating pressure. This enables optimal use of shorter length and smaller diameter sub-2µm particle columns, implementation of high throughput ballistic gradient methods, and assures predictable system performance across a range of operating pressures and flow rates to make the most of small particle technology. The ACQUITY UPLC System’s sample-handling addresses the unique challenges of UPLC with: standardization on SBS-compliant plates; user-controlled sample temperature; programmable injection protocols for managing throughput and carryover. And when interfaced with the Sample Organizer, it increases unattended sample capacity by ten times. High-speed detectors, both optical and mass, contribute to increased sensitivity and help manage the heightened speed and resolution requirements of UPLC. ACQUITY UPLC Systems are easily controlled, diagnosed, and monitored via a graphical system console interface. The console offers:
1. Quick and easy access to critical instrument parameters
2. Simple system start-up, elegant system status monitoring and predictive performance indicators to ensure maximum productivity
3. Data management capabilities enabled by MassLynxTM and EmpowerTM software
UPLC Optical and Mass Detection:
1. ACQUITY UPLC Fluorescence (FLR) Detector
2. ACQUITY UPLC Photodiode Array (PDA) Detector
3. ACQUITY UPLC Evaporative Light Scattering (ELS) Detector
4. ACQUITY UPLC Tunable UV (TUV) Detector
5. From high-performance single quad mass detectors to advanced tandem and time-of-flight (Q-Ttof) mass spectrometers.
6. The ACQUITY UPLC System is easily compatible with third-party MS solutions (ABI/MDS SCIEX, Bruker Daltonics, and Thermo Fisher Scientific)
Contact: Waters
http://www.waters.com
CIRCULAR DICHROISM DETECTOR FOR ULTRA HIGH PRESSURE LIQUID CHROMATOGRAPHY Chiral separation has become one of the most important application areas in HPLC, including the use of ultra-high pressure HPLC (X-LC®). The increasing demand for chiral chromatography in various fields, such as drug analysis, drug discovery, biochemical analysis, natural product analysis, organic synthesis, etc. have resulted in a parallel demand for optimized chiral separations and more sensitive detection methods. Recent advances in chiral column technology are boosting the use of chiral chromatography in a greater number of laboratories; however, advances in chiral detection technology have only recently met the required demands in terms of sensitivity and selectivity. Circular Dichroism (CD) is based upon the differential absorption of right and left circularly polarized light as a result of a chiral chromaphore and is a much more specific technique than Optical Rotation (OR) or Polarimetry techniques. CD detectors can provide a negative or positive signal based on the chirality of the eluting molecule, thus providing positive identification of the molecular chirality, unlike conventional UV, RI or Fluorescence detectors which cannot differentiate between the chiral molecules. JASCO developed the new 3195CD Circular Dichroism Chiral Detector as an innovative CD detector for HPLC systems including the JASCO X-LC systems, using the same technology applied in conventional CD spectropolarimeters. The 3195CD enables highly sensitive and selective detections of chiral compounds, simultaneously determining both the CD and UV absorption of the sample in the same cell, and determines optical isomer separation and purity. To meet X-LC requirements, the 3195CD features a high speed sampling rate of 50 data points/sec, for both the CD and UV signals, and the specially designed flow cell minimizes peak broadening.
Contact: JASCO Inc.
http://www.jascoinc.com
Ultra Fast Liquid Chromatograph Shimadzu Scientific Instruments’ new Prominence Ultra Fast Liquid Chromatograph (UFLC) system is up to 10 times faster than a conventional HPLC system using a 5-µm particle column. Key to the system is the SIL-20A autosampler, capable of a 10-s injection cycle while delivering ≤ 0.3% RSD area repeatability. Prominence UFLC successfully reduces carryover by inhibiting the adsorption of samples through precise engineering and the prudent choice of wetted materials in the flow path. With Shim-pack XR Series columns, Prominence UFLC can provide high-speed, high-resolution analysis at pressures below 30MPa (300 kgf/cm2). The columns are available with inner diameters of 2, 3, or 4.6 mm and lengths of 30, 50, 75, or 100 mm, allowing FAST LC to be performed on conventional HPLC equipment. With its micro-plunger design, the LC-20AD solvent-delivery system provides unmatched gradient resolution and reproducibility, and has a delivery range of 100 nL/min to 10 mL/min. Prominence UFLC is versatile and can accept an assortment of flow-control valves and a column-switching system.
Contact: Shimadzu Scientific Instruments
http://www.ssi.shimadzu.com
