Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
48, Number 1, January 2010, pp. 76-80
Simultaneous Analysis of Xanthone
Glycosides in Halenia elliptica by HPLC–DAD-ESI-MS
Xia Liu1,2, Yong Liu1,2, Juan Chen1,
and Yan-Ping Shi1
1Key Laboratory for Natural Medicine of Gansu Province, Lanzhou
Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou,
P.R. China and
2Graduate University of Chinese Academy of Sciences,
Beijing, P.R. China
A new, simple, and sensitive high-performance
liquid chromatography–diode array detector (HPLC–DAD)
method was developed for the simultaneous determination of six
major xanthone glycosides in Halenia elliptica. The
chemical profile of six xanthone glycosides, including 2,3,5-trimethoxy-1-O-primeverosyloxyxanthone
(Analyte 1), 2,3,4,5-tetramethoxy-1-O-primeverosyloxyxanthone
(Analyte 2), 2,3,5,7-tetramethoxy-1-O-primeverosyloxyxanthone
(Analyte 3), 2,3,7-trimethoxy-1-O-primeverosyloxyxanthone (Analyte
4), 2,3,4,7-tetramethoxy-1-O-primeverosyloxy-xanthone (Analyte
5), and 2,3,4,5,7-pentamethoxy-1-O-primeverosyloxy-xanthone (Analyte
6) were acquired by using HPLC–DAD coupled to an electrospray
ionization mass spectrometer. The analysis was performed on a
Kromasil C18 column (5 µm, 250 ×4.6 mm
i.d.), using acetonitrile–H2O (25:75, v/v) as
the mobile phase at a flow rate of 0.8 mL/min. Under UV detection
at 260 nm, the recoveries of the analytes were in the range of
96.1–100.3%, the LODs
were within 0.20 µg/mL, and all the xanthone glycosides
showed good linearity (r ≥ 0.9990) in a relatively wide concentration
range; the intra-day relative standard deviations (%) ranged
from 0.65 to 1.39%, and the inter-day RSDs% were not higher than
5%. The proposed method is suitable for quantitative and qualitative
determination of the xanthone glycosides in H.
elliptica.
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