Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
48, Number 1, January 2010, pp. 68-75
Selective Densitometric Determination of Four
α-Aminocephalosporins Using Ninhydrin Reagent
Gamal A. Saleh, Fardous A. Mohamed, Salwa R. El-Shaboury,
and Azza H. Rageh
Department of Pharmaceutical Analytical Chemistry, Facutly of
Pharmacy, Assiut Univeristy, 71526 Assiut, Egypt
A simple, selective, and precise densitometric
method for analysis of four α-aminocephalosporins, namely cefaclor
monohydrate, cefadroxil monohydrate, cefalexin anhydrous, and
cefradine anhydrous, both in bulk drugs and in formulations was
developed and validated. The method employed thin-layer chromatography
(TLC) aluminium sheets precoated with silica gel G 60 F254 as
the stationary phase. The solvent system consists of ethyl acetate–methanol–water
with different ratios for all studied drugs (Rf values
of 0.40–0.60).
The separated spots were visualized as blue to violet color after
spraying with ninhydrin reagent. The linear regression analysis
data for the calibration plots of all studied drugs produced
a good linear relationship with correlation coefficients ranging
from 0.9990 to 0.9996 and coefficients of determination ranging
from 0.9986 to 0.9992 over the concentration range 2–10 µg/spot.
The limits of detection and quantitation for all studied drugs
ranged from 0.09 to 0.23 and from 0.27 to 0.84 µg/spot,
respectively. The developed method was applied successfully for
the determination of the studied drugs in their pharmaceutical
dosage forms with good precision and accuracy. Also, the method
can be employed as a promising stability-indicating assay.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To
order electronically
click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers. |
Site Map: Home
| Current Issue | Subscribe
| Back Issues | About
Us | Meetings | Advertising
|
| Books for Sale | For
the Author | Links | Supplier
Info | Search | |
|
