Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
48, Number 1, January 2010, pp. 63-67
Development and Validation of a Confirmatory
HPLC Method for Simultaneous Determination of Sudan Dyes in Animal
Tissues and Eggs
Yin-Liang Wu1,3, Cun Li2, Xi Xia1,
Yong-Jun Liu1,3, and
Jian-Zhong Shen1,
1College of Veterinary Medicine, China Agricultural University,
Beijing 100094, People’s Republic of China;
2College of Agriculture,
Heibei University of Engineering, Handan 056038, People’s Republic
of China; and
3Quality Control and Inspection Center for Domestical Animal
Products of MOA, Beijing 100026, People’s Republic of China
A simple and sensitive high-performance liquid
chromatography (HPLC) analytical method for the simultaneous
determination of six Sudan dyes (Sudan Red G, Sudan I, Sudan
II, Sudan III, Sudan Red 7B, Sudan IV) in animal tissues and
eggs was developed. Samples were extracted with acetonitrile
followed by a cleanup using a C18 solid-phase extraction
column. Chromatographic separation was achieved on a Zorbax SB-C18 column
under gradient conditions. The analytes were detected at 510
nm by HPLC with diode array detection. Specificity, decision
limit (CCα), detection capacity (CCβ), accuracy, and
precision were determined during validation process. Recoveries
for six Sudan dyes from three animal tissues and eggs were 77.2–98.0%
with excellent relative standard deviations. CCα and CCβ were
in the range of 7.7–9.0 μg/kg and 9.1–10.3 μg/kg,
respectively. The limits of quantitations were between 12.8 μg/kg
and 15.0 μg/kg.
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