Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
48, Number 1, January 2010, pp. 59-62
Simultaneous Quantitative Determination of Cinnamaldehyde
and Methyl Eugenol From Stem Bark of Cinnamomum
zeylanicum Blume
Using RP-HPLC
Atish Gursale, Vidya Dighe, and Guarang Parekh
Ramnarain Ruia College, Department of Chemistry, Mumbai, India
A simple, sensitive, and precise reversed-phase
high performance liquid chromatographic (HPLC) method has been
developed, validated and used for simultaneous quantitative determination
of cinnamaldehyde and methyl eugenol from the methanolic extract
of dried bark powder of Cinnamomum zeylanicum Blume
(family Lauraceae). The ultrasonic extraction method was used
for the extraction of these compounds. The reversed-phase HPLC
analysis was carried out using a Intersil ODS-3V-C18 (150
mm × 4.6
mm, 5 µm)
column and a mobile phase comprising of methanol–acetonitrile–water
in the volume ratio of 35:20:45, delivered at a flow rate of
1.0 cm3/min. The detection and quantitation of both
the compounds was carried out at 221 nm.
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