Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
48, Number 1, January 2010, pp. 49-54
Simultaneous Determination of Tetracaine, Proline,
and Enoxacin in Human Urine by CE with ECL Detection
Hanwen Sun1, Ming Su1, and Liqing
Li1,2,
1College of Chemistry and Environmental Science, Hebei University,
Key Laboratory of Analytical Science and Technology of Hebei Province,
Baoding, 071002, China and
2Department of Chemistry, Taishan University,
Taian, 271021, China
A simple, fast, and sensitive capillary electrophoresis
method was developed for the simultaneous determination of tetracaine,
proline, and enoxacin in human urine with electrochemiluminescence
(ECL) detection. The effects of experimental conditions including
the mode of applied voltage signal, the potential of working
electrode, pH value, the flow rate of carrier of solution, the
concentration of Ru(bpy)32+, and the ECL
intensity of the drugs were investigated in detail. Parameters
related to the separation and detection were investigated and
optimized. Under optimized conditions, the proposed method displayed
a linear range from 0.4 to 100 µg/mL for tetracaine, 0.2
to 80 µg/mL
for proline, and 0.1 to 100 µg/mL for enoxacin. Their limits
of detection were 0.08, 0.06, and 0.02 µg/mL, respectively.
A baseline separation for tetracaine, proline, and enoxacin was
achieved within 10 min. Developed method was successfully applied
to determine tetracaine, proline, and enoxacin in human urine.
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