Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
48, Number 1, January 2010, pp. 22-25
Determination of Para Red, Sudan Dyes, Canthaxanthin,
and Astaxanthin in Animal Feeds Using UPLC
Xiaolin Hou1,Yonggang Li2, Guojuan
Wu1, Lei Wang3, Miao
Hong4, and Yongnin Wu4,
1Department of Animal Science, Beijing University of Agriculture,
Beijing, P.R. China, 102206;
2Circulation Industry Promotion Center of
Ministry of Commerce, Beijing, 100070;
3Department of Preventative Medicine,
College of Veterinary Medicine, China Agricultural University, 100094;
4Institute of Nutrition and Food Hygiene, Chinese Center for
Disease Control and Prevention, 100050
A simple high-performance liquid chromatography
method was developed for quantitative determination of para red,
Sudan I, Sudan II, Sudan III, Sudan IV, canthaxanthin, and astaxanthin
in feedstuff. The sample was extracted using acetontrile and
cleaned up on a C18 SPE column. The residues were
analyzed using ultra-performance liquid chromatography coupled
to a diode array detector at 500 nm. The mobile phase was acetonitrile–formic
acid–water with a gradient elution condition. The external
standard curves were calibrated. The mean recoveries of the seven
colorants were 62.7–91.0% with relative standard deviation
2.6–10.4% (intra-day) and 4.0–13.2% (inter-day).
The detection limits were in the range of 0.006–0.02 mg/kg.
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