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Published:Journal of Chromatographic Science, ISSN 0021-9665 Volume 48, Number 1, January 2010, pp. 18-21

Applications of Ultra-Performance Liquid Chromatography to Traditional Chinese Medicines

Gui-Ling Yang1, Li-Wei Yang2, Yong-Xue Li2, Hui Cao1, Wen-Liang Zhou1, Zhi-Jian Fang3, Hong-Bo Zhou3, Jie-Li Mo2, Shu-Xiong Xiao2, and Hui-Rong Lin2
1School of Life Science, Sun Yat-Sen University, Guangzhou, 510275, China;
2Guangdong Institute for Drug Control, Guangzhou, 510180, China;
3Guangdong Pharmaceutical University, 510006, China

Ultra-performance liquid chromatography (UPLC), as a development of the chromatographic technique, was used in the quality control of Panax ginseng C.A. Mey. In the study, the UPLC methods were transferred from conventional high-performance liquid chromatography (HPLC) methods according to chromatographic equations. The results demonstrated that UPLC analysis methods could be transferred from HPLC without loss of efficiency. Compared with conventional HPLC, UPLC made a surprising 10-fold increase in speed and 20-fold decrease in solvent consumption. The study indicated UPLC as a suitable alternative to HPLC and can be reliably applied to quality control of traditional Chinese medicines.

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