Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
48, Number 1, January 2010, pp. 12-17
UHPLC Separation with MS Analysis for Eight Carbonyl
Compounds in Mainstream Tobacco Smoke
John H. Miller IV, William P. Gardner, and Ricardo R.
Gonzalez
Altria Client Services, Research Development & Engineering, 601 E.
Jackson Street, Richmond, VA 23219, USA
A method to quantify eight carbonyl compounds
in mainstream cigarette smoke is presented using ultra-high pressure
liquid chromatography (UHPLC). The combination of UHPLC and mass
spectrometry (UHPLC–MS) dramatically reduces analysis times
as compared to the current in-house high-performance liquid chromatography
(HPLC)–UV method. In addition, improved detector selectivity
and peak resolution are observed. Sample analysis times are reduced
from 47 min with HPLC–UV to less than 5 min using this
improved method. Atmospheric pressure chemical ionization, atmospheric
pressure photo ionization, and electrospray ionization are directly
compared to evaluate ionization potential and linear response
range for the carbonyl 2,4-dinitrophenylhydrazine derivatives.
Smoke extracts from three standard smoking protocols are analyzed
by both UHPLC–MS and HPLC–UV for method comparison
purposes.
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