Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 5, May/June 2009, pp. 387-391
Development of an Analytical Methodology for
Simultaneous Determination of Vincristine and Doxorubicin in
Pharmaceutical Preparations for Oncology by HPLC–UV
Ana Sofia Rodrigues1, Ana Rita Lopes1,
Ana Leão1,
Alda Couceiro1, Ana Bela Sarmento Ribeiro2,3,
Fernando Ramos1,
Maria Irene Noronha da Silveira1, and Catarina Resende
de Oliveira2 1Grupo de Bromatologia—Centro de Estudos Farmacêuticos,
Faculdade de Farmácia da Universidade de Coimbra, 3000-295
Coimbra, Portugal and 2Instituto de Bioquímica e Biologia
Molecular, Faculdade de Medicina da Universidade de Coimbra,
3000-295 Coimbra, Portugal, and 3CIMAGO—Centre for Research
of Environment, Genetics, and Oncobiology, 3001-301 Coimbra,
Portugal
A high-performance liquid chromatography–UV
methodology (λ = 230 nm) was developed and validated for
the simultaneous determination of vincristine and doxorubicin
in pharmaceutical preparations used in oncology. The chromatography
was carried out on a C18 column using acetonitrile
90% in water–potassium
hydrogenphosphate buffer 50mM, pH 3.2 ± 0.1 (32:68, v/v)
as mobile phase at a flow rate of 1.5 mL/min. The method proved
to be specific, exact, and accurate, in accordance with the ICH
standards, presenting linearity in the 1–5 µg/mL
and 5–100 µg/mL intervals, and detection (0.19 × 0.51 µg/mL)
and quantification (0.63 × 1.7 µg/mL) limits for
vincristine and doxorubicin, respectively.
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