Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 5, May/June 2009, pp. 345-348
Separation of Five Isomers of Dihydroxybenzoic
Acid by High-Speed Counter-Current Chromatography with Dual-Rotation
Elution Method
Dan Liu1, Zhiguo Su2, Changhai
Wang1, and Ming
Gu2, 1School of Environmental and Biological Science and Technology,
Dalian University of Technology and 2National Key Laboratory
of Biochemical Engineering, Institute of Process Engineering,
Chinese Academy of Sciences
Five small molecular isomers, 2,3-, 2,4-, 2,6-,
3,4-, and 3,5-dihydroxybenzoic acid, were successfully separated
in one step with solvent system n-hexane–ethyl
acetate–methanol–water
(1:5:1.5:5) on high-speed counter-current chromatography (HSCCC).
A new method, dual-rotation elution, was successfully used to
decrease separation time and increase resolution. Five peak factions
were eluted within 850 min. HSCCC became an efficient method
to separate small molecular isomers from intermediates and products
of organic synthesis, especially with the dual-rotation elution
method.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
For permission to reuse material from Journal of Chromatographic Science, please access www.copyright.com or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of uses.
