Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 5, May/June 2009, pp. 341-344
Purification of Rutin and Nicotiflorin from the
Flowers of Edgeworthia chrysantha Lindl. by High-Speed
Counter-Current Chromatography
Shengqiang Tong1, Jizhong Yan1,
Gang Chen2,
and Jianzhong Lou3 1College of Pharmaceutical Science, Zhejiang University of Technology,
Hangzhou, 310032, China; 2Xinchang Pharmaceutical Factory, Zhejiang
Medicine Co. Ltd., Shaoxing, 312500, China; and 3Department of
Chemical Engineering, North Carolina Agricultural and Technical
State University, Greensboro, NC 27411
An ethanol extract of air-dried flowers of Edgeworthia
chrysantha Lindl. was partitioned between water and petroleum,
ethyl acetate, and n-butanol. The n-butanol
extraction was initially purified by silica gel column chromatography
to give a partially purified sample. The bioactive compound rutin,
along with nicotiflorin, were successfully separated from the
partially purified sample by high-speed counter-current chromatography.
The two compounds were isolated from the plant of Edgeworthia genus
for the first time. The two-phase solvent system used was composed
of ethyl acetate–n-butanol–water at an optimized
ratio of 4:1:5 (v/v/v). High-speed counter-current chromatography
yielded, from 108 mg of the partially purified extract, 53 mg
rutin and 32 mg nicotiflorin with 92.5% and 92.2% recovery, with
each at over 96.5% purity by high-performance liquid chromatography
analysis. Their structures were identified by 1H NMR
and 13C NMR.
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