|
Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 5, May/June 2009, pp. 333-336
Preparative Purification of Morroniside and Loganin
from Fructus corni by Combination of Macroporous Absorption
Resin and HSCCC
Liangpo Liu, Ailing Sun, Sujuan Wu,
and Renmin Liu
College of Chemistry and Chemical Engineering, Liaocheng University,
Liaocheng, 252059, PR China
A method for preparative purification of loganin
and morroniside from Fructus corni was established by
combination of macroporous absorption resin column separation
and high-speed counter-current chromatography (HSCCC). The crude
extracts obtained from Fructus corni by ultrasonic extraction
with 50% methanol were subjected to separation on a macroporous
resin column and then eluted with 15% and 40% ethanol, respectively.
A fraction of 40% ethanol was used as the sample for separation
of morroniside and loganin by HSCCC. The two-phase solvent system
used for HSCCC separation was n-butanol–methanol–1%
acetic acid water (4:1:6, v/v). The upper phase was used as the
stationary phase of HSCCC. Morroniside (28.7 mg) of 97.8% purity
and loganin (11.5 mg) of 98.6% purity were obtained in a one-step
HSCCC separation from 50 mg of sample. The structures of morroniside
and loganin were identified by 1H-NMR and 13C-NMR.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted. To
order electronically
click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
To order multiple copies click here.
Please
indicate JCS volume and issue along with
page numbers. |
 For permission to reuse material from Journal of Chromatographic Science, please access www.copyright.com or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of uses.
Site Map: Home
| Current Issue | Subscribe
| Back Issues | About
Us | Meetings | Advertising
|
| Books for Sale | For
the Author | Links | Supplier
Info | Search | |
