Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 5, May/June 2009, pp. 329-332
Separation and Purification of Rutin and Acaciin
from the Chinese Medicinal Herb Herba Cirsii by Combination
of Macroporous Absorption Resin and High-Speed Counter-Current
Chromatography
Li Meng, Renmin Liu, Ailing Sun, Sujuan Wu,
and Ningnig Liu
College of Chemistry and Chemical Engineering, Liaocheng University,
Liaocheng, 252059
A preparative high-speed counter-current chromatography
(HSCCC) method for isolation and purification of rutin and acaciin
from the Chinese medicinal herb Herba Cirsii was successfully
established. The crude extracts obtained from Herba
Cirsii by
water under reflux were subjected to a macroporous resin column
and eluted with 10% and 60% ethanol, respectively. The fraction
of 60% ethanol was used as the sample for HSCCC separation of
rutin and acaciin. The two-phase solvent system used for the
separation was ethyl acetate–n-butanol–water
(5:1.5:5, v/v) and the upper phase was used as the stationary
phase. Rutin (25.2 mg) and acaciin (21.8 mg) with a purity of
99.2% and 99.6%, respectively, were purified successfully from
60 mg of sample. The chemical structures of rutin and acaciin
were identified by 1H-NMR and 13C-NMR.
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