Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 3, March 2009, pp. 211-215
Development of a Reversed-Phase High-Performance
Liquid Chromatographic Method for Analyzing Furanocoumarin Components
in Citrus Fruit Juices and Chinese Herbal Medicines
Ying-Ku Lin, Ming-Thau Sheu, Chia-Hui Huang,
and Hsiu-O Ho
College of Pharmacy, Taipei Medical University, 250 Wu-Hsing
Street, Taipei 110, Taiwan (ROC)
A rapid and sensitive reversed-phase high performance
liquid chromatographic method for the quantitation of five furanocoumarins
(bergaptol, psoralen, bergapten, 6',7'-dihydroxybergamottin,
and bergamottin) is developed and validated. HPLC analysis of
these five furanocoumarins is performed on a reversed-phase Inertsil
ODS-2 column with a particle size of 5 µm. Using only water
and acetonitrile as solvents, good separation, good precision,
and high accuracy are obtained for the analysis of furanocoumarin
components. This method is validated and applied to analyze the
composition of five furanocoumarins in four citrus fruit juices
(grapefruit, pomelo I, pomelo II, and shaddock) and ten Chinese
herbal medicines (Bai-Zhi, Qiang-Huo, Du-Huo, Fang-Feng, Dang-Gui,
Huang-Qin, Gan-Cao, Chen-Pi, Ge-Gen, and Yin-Chen-Hao) prepared
by water decoction or an alcohol infusion. Results show that
four of the five furanocoumarins (but not bergapten) are detected
in grapefruit, pomelo I, and pomelo II, and the highest amount
of these components is found in grapefruit juice. In the ten
Chinese herbal medicines, the five furanocoumarins are not detected
in Ge-Gen or Yin-Chen-Hao. The remaining herbs contain various
compositions and amounts of furanocoumarins. In general, Chinese
herbal medicines prepared by the 40% ethanol infusion contain
larger amounts of furanocoumarins than those prepared by hot
water decoction.
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