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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 6, July 2009, pp. 492-496
?Electrochromatographic Evaluation of Diol-Bonded
Silica Monolith Capillary Column for Separation of Basic Compounds
?Fanggui Ye, Shuting Li, and Shulin Zhao
College of Chemistry and Chemical Engineering, Guangxi Normal
University, Guilin 541004, China
?A diol-bonded silica monolith capillary column
as polar stationary phase was successfully prepared for capillary
electrochromatography. The preparation of monolithic stationary
phase was based on the individual silica matrix forming and subsequent
chemical bonding by (3-glycidoxypropyl) trimethoxysilane to produce
the desired function. The diol-bonded silica monolith has been
successfully employed in the electrochromatographic separation
of alkaloids. The effects of experimental parameters, such as
the volume fraction of the organic modifier, pH value, and ionic
strength of the buffer on the retention behavior of the solutes
were investigated. Column efficiencies greater than 110,000 plates/m
for separation of basic compounds were obtained. It was observed
that retention of alkaloids on the diol-bonded silica monolith
was mainly contributed to a reversed-phase and cation-exchange
mechanism, and electrophoresis of basic compounds also played
a role in separation.
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