Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 6, July 2009, pp. 485-491
Simultaneous Determination of Three Antioxidants
in Foods and Cosmetics by Flow Injection Coupled to an Ultra-short
Monolithic Column
J.F. García-Jiménez, M.C. Valencia, and
L.F. Capitán-Vallvey
Solid Phase Spectrometry Research Group, Department of Analytical
Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada,
E-18071 Granada, Spain.
The combination of an ultra-short C18 monolithic
column (5 mm long) with flow injection analysis results in a
versatile and efficient system that has been used for the determination
of three antioxidants [propylgallate (PG), butylhydroxyanisole
(BA), and butylhydroxytoluene (BT)]. Due to the wide variety
of polarities of the analytes, two different carriers (carrier
A: methanol–water 42% and carrier B: methanol–water
70%) were able to separate the analytes in only 85 s. The applicable
concentration range, the detection, and the relative standard
deviation (n = 10) were: for PG, from 2.77 to 300 µg/mL,
0.84 µg/mL, 2.84%; for BA, between 1.51 and 300 µg/mL,
0.46 µg/mL, and 2.70%; and for BT, between 1.65 and 100 µg/mL,
0.55 µg/mL, and 2.22%, respectively. The method was applied
and validated satisfactorily for the determination of PG, BA,
and BT in food and cosmetic samples.
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