Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 1, January 2009, pp. 92-96
Identification of N-Nitrosamines in Treated
Drinking Water Using Nanoelectrospray Ionization High-Field Asymmetric
Waveform Ion Mobility Spectrometry with Quadrupole Time-of-Flight
Mass Spectrometry
Yuan Yuan Zhao1, Xin Liu2,
Jessica M. Boyd1,
Feng Qin1, Jianjun Li2, and Xing-Fang Li1, 1Division of Analytical and Environmental Toxicology, Department
of Laboratory Medicine and Pathology, University of Alberta,
10-102 Clinical Sciences Building, Edmonton, Alberta, Canada
T6G 2G3 and 2Institute for Biological Sciences, National Research
Council of Canada, 100 Sussex Drive, Ottawa, Ontario, Canada
K1A OR6
We report a nanoelectrospray ionization (nESI)
with high-field asymmetric waveform ion mobility spectrometry
(FAIMS) and tandem mass spectrometry (MS–MS) method for
determination of small molecules of m/z 50 to 200 and its potential
application in environmental analysis. Integration of nESI with
FAIMS and MS–MS combines the advantages of these three
techniques into one method. The nESI provides efficient sample
introduction and ionization and allows for collection of multiple
data from only microliters of samples. The FAIMS provides rapid
separation, reduces or eliminates background interference, and
improves the signal-to-noise ratio as much as 10-fold over nESI-MS–MS.
The tandem quadrupole time-of-flight MS detection provides accurate
mass and mass spectral measurements for structural identification.
Characteristics of FAIMS compensation voltage (CV) spectra of
seven nitrosamines, N-nitrosodimethylamine (NDMA), N-nitrosomethylethylamine
(NMEA), N-nitrosodiethylamine (NDEA), N-nitrosodi-n-propylamine
(NDPA), N-nitrosodi-n-butylamine (NDBA), N-nitrosopiperidine
(NPip), and N-nitrosopyrrolidine (NPyr), were analyzed. The optimal
CV of the nitrosamines (at DV –4000 V) were: –1.6
V, NDBA; 2.6 V, NDPA; 6.6 V, NPip; 8.8 V, NDEA; 13.2 V, NPyr;
14.4 V, NMEA; and 19.4 V, NDMA. Fragmentation patterns of the
seven nitrosamines in the nESI-FAIMS-MS–MS were also obtained.
The specific CV and MS–MS spectra resulted in positive
identification of NPyr and NPip in a treated water sample, demonstrating
the potential application of this technique in environmental
analysis.
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