Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 1, January 2009, pp. 44-51
Evaluation of Derivatization Strategies for the
Comprehensive Analysis of Endocrine Disrupting Compounds using
GC/MS
John A. Bowden1, Dominic M. Colosi1,
Diana C. Mora-Montero1, Timothy J. Garrett2,
and Richard A. Yost1, 1Department of Chemistry, University of Florida, PO Box 117200,
Gainesville, FL 32611 and 2Biomedical Mass Spectrometry Laboratory,
Department of Medicine, PO Box 100322, Gainesville, FL 32610
The expanding list of endocrine disrupting compounds
(EDCs) has increased the need for the development of improved
monitoring methods to evaluate exposure. Furthermore, the diverse
physiochemical properties of EDCs impose inherent analytical
limitations, and, thus, a new comprehensive method that can simultaneously
analyze numerous EDCs in one chromatographic analysis would be
a significant improvement over current EDC detection methods.
Gas chromatography/mass spectrometry (GC/MS) offers promising
profiling capabilities; however, many polar EDCs require derivatization
for adequate detection. Here, a novel method for the comprehensive
profiling of EDCs that employs a silyl derivatization strategy
to expand the polarity range of compounds able to be separated
and detected in a single chromatographic analysis is presented.
The comprehensive method successfully separates 21 GC-ready and
12 derivatization-required EDCs in one chromatographic analysis.
Thermal and microwave derivatization methods are effective for
a comprehensive EDC mixture, although the microwave derivatization
often proves more effective in shorter analysis time. A pilot-study
of spiked surface water from Lake Apopka (Florida) demonstrates
the potential of the comprehensive EDC profiling method.
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