Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 2, February 2009, pp. 164-169
Sensitive Liquid Chromatography Positive Electrospray
Tandem Mass Spectrometry Method for the Quantitation of Tegaserod
in Human Plasma Using Liquid–Liquid Extraction
Ramakrishna Nirogi, Vishwottam Kandikere, and
Koteshwara Mudigonda
Biopharmaceutical Research, Suven Life Sciences Ltd., Serene
Chambers, Road -5, Avenue -7, Banjara Hills, Hyderabad 500034,
India
A sensitive and rapid high-performance liquid
chromatography–positive ion electrospray tandem mass spectrometry
method is developed and validated for the quantitation of tegaserod
in human plasma. Following liquid–liquid extraction, the
analytes are separated using an isocratic mobile phase on a reversed-phase
column and analyzed by tandem mass spectrometry in the multiple
reaction monitoring mode using the respective (M+H)+ ions, m/z
302 to 173 for tegaserod and m/z 409 to 228 for the
internal standard. The assay exhibits a linear dynamic range
of 100–10000
pg/mL for tegaserod in human plasma. The lower limit of quantitation
is 100 pg/mL with a relative standard deviation of less than
7%. Acceptable precision and accuracy are obtained for concentrations
over the standard curve range. A run time of 2.0 min for each
sample makes it possible to analyze more than 250 human plasma
samples per day. The validated method is successfully used to
analyze human plasma samples for application in pharmacokinetic,
bioavailability, or bioequivalence studies.
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