Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 7, August 2009, pp. 585-588
A Simple Method for Screening and Quantification
of Ricinine in Feed with HPLC and LC–MS
Zongyi Wang1,2, Defa Li1, Zhiqiang
Zhou2, Bingying
Li1, and Wenjun Yang1
1Ministry of Agriculture Feed Safety and Bio-availability Evaluation
Center, China Agricultural University, Beijing, P.R. China;
2Department
of Applied Chemistry, China Agricultural University, Beijing, P.R. China
A simple high-performance liquid chromatography
(HPLC) and liquid chromatography–mass spectrometry (LC–MS)
method for screening and quantification of ricinine in feeds
is established for use in feed quality control. Ricinine is extracted
from the sample with methanol. The sample extract is directly
used for screening with LC–MS and is cleaned up using solid-phase
extraction for quantification with HPLC and LC–MS. A 9
min isocratic elution with 10% acetonitrile (v/v) at a flow rate
of 0.2 mL/min is performed on an Atlantis dC18 column
(5 µm,
2.1 × 100 mm) at ambient temperature with UV detection
at 310 nm and MS in positive selective ion recording mode. Good
linearity (r2 ≥ 0.99) is found in the
investigated concentration ranges from 0.054 to 55.4 µg/mL
for HPLC and 5 to 1000 ng/mL for LC–MS. In the final extracts,
the limits of detection (LOD) and quantification (LOQ) for HPLC
are 75 pg and 150 pg, respectively; the LOD and LOQ for HPLC–MS
are 6 pg and 15 pg, respectively.
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