Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 7, August 2009, pp. 581-584
High-Performance Liquid Chromatographic Method
for the Determination of Cyromazine and Melamine Residues in
Milk and Pork
Ruicheng Wei1, Ran Wang1, Qingfei
Zeng2, Ming Chen1,
and Tiezheng Liu1,
1Jiangsu Province Key Laboratory of Animal-Derived Food Safety,
Jiangsu Academy of Agricultural Science, Jiangsu, Nanjing 210014, P.R.
China;
2State Key laboratory of Lake Science and Environment, Nanjing
Institute of Geography and Limnology and the Graduate School of Chinese
Academy of Sciences, Nanjing 210008, P.R. China TP
A high-performance liquid chromatography method
is described in this paper. The method uses NH2 column
and 97% acetonitrile eluate to determine the insecticide cyromazine
and metabolite melamine residues in milk and pork. Samples were
treated with NaOH and extracted with acetonitrile containing
20% NH4OH. Target analytes of samples were cleaned up and concentrated
by C18 column solid-phase extraction. A separation
for cyromazine and melamine was achieved, and respective retention
times were 8 and 12 min. The calibration curves for cyromazine
and melamine were linear in a concentration range of 0.01–1.0 µg/mL,
with correlation coefficients of 0.9999 and 0.9997, respectively.
The limit of detection of both compounds was 0.2 ng, and the
limit of quantitation was 0.02 mg/kg. Recoveries of cyromazine
and melamine at fortified levels of 0.02, 0.05, and 0.1 mg/kg
ranged from 84.5–90.8%, and 83.6–91.3%, respectively,
with coefficient of variation of 3.1–7.8%.
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