Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 7, August 2009, pp. 564-568
Determination of γ-Aminobutyric Acid in
Food Matrices by Isotope Dilution Hydrophilic Interaction Chromatography
Coupled to Mass Spectrometry
Raniero Zazzeroni, Andrew Homan, and Emma Thain
Unilever, Safety and Environmental Assurance Centre, Colworth
Science Park, Sharnbrook, MK44 1LQ, United Kingdom
The estimation of the dietary intake of γ-aminobutyric
acid (GABA) is dependent upon the knowledge of its concentration
values in food matrices. To this end, an isotope dilution liquid
chromatography–mass spectrometry method has been developed
employing the hydrophilic interaction chromatography technique
for analyte separation. This approach enabled accurate quantification
of GABA in apple, potato, soybeans, and orange juice without
the need of a pre- or post-column derivatization reaction. A
selective and precise analytical measurement has been obtained
with a triple quadrupole mass spectrometer operating in multiple
reaction monitoring using the method of standard additions and
GABA-d6 as an internal standard. The concentrations
of GABA found in the matrices tested are 7 µg/g of apple,
342 µg/g
of potatoes, 211 µg/g of soybeans, and 344 µg/mL
of orange juice.
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