Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 7, August 2009, pp. 534-539
Evaluation of Methods for the Simultaneous Analysis
of Cations and Anions Using HPLC with Charged Aerosol Detection
and a Zwitterionic Stationary Phase
Christopher Crafts, Bruce Bailey, Marc Plante, and Ian
Acworth
ESA Biosciences, Inc., 22 Alpha Road, Chelmsford, MA 01824
This paper describes the development and qualification
of a method capable of analyzing inorganic ions as salts and
counter-ions of both active pharmaceutical ingredients and other
compounds such as lysine. The use of a polymeric zwitterionic
column with a binary high-performance liquid chromatography gradient
enables the separation of several anions and cations in a single
run. A generic gradient (method #1) was developed and validated
with respect to specificity, correlation, intermediate precision,
accuracy, and sensitivity (limits of quantitation and detection)
for four anions and two cations. Furthermore, the ability to
alter chromatographic selectivity by simple gradient manipulation
(without altering the mobile phase composition or column type)
is demonstrated for nine anions and three cations (method #2).
The simultaneous measurement of cations and anions at the parts
per billion level using the Corona charged aerosol detector with
zwitterionic chromatography–polymeric hydrophilic interaction
chromatography is a viable alternative to traditional techniques
used for ion analysis.
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