Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 4, April 2009, pp. 324-328
Simultaneous Determination
of Salbutamol, Ractopamine, and Clenbuterol in Animal Feeds by
SPE and LC–MS
L.Y. Zhang, B.Y. Chang, T. Dong, P.L. He, W.J.
Yang, and Z.Y. Wang
State Key Laboratory on Animal Nutrition, China Agricultural
University, Beijing P. R. China, 100094
A liquid chromatography–mass spectrometry
(LC–MS) method for the simultaneous determination of salbutamol,
ractopamine, and clenbuterol in commercial feeds was developed.
Samples were extracted with phosphoric acid–methanol solution,
and further clean-up was achieved with a C18 cation exchange
mixed mode cartridge. Separation of analytes was developed on
a C18 column with 0.01M aqueous ammonium formate solution (pH
3.8)–acetonitrile by gradient program, and characterized
by LC–MS on a quadrupole detector, in electrospray positive
ion mode. This method provides average recoveries for salbutamol,
ractopamine, and clenbuterol of 83–110% and coefficients
of variation of 1.5–11% in feeds spiked in the range of
0.5–500 mg/kg. The limits of detection and quantification
in feeds were 0.01 mg/kg and 0.05 mg/kg, respectively. Such limits
are well below the dose of 2–25 mg/kg feed proposed as
effective.
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