Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 4, April 2009, pp. 272-278
GC Determination of Acetone, Acetaldehyde, Ethanol,
and Methanol in Biological Matrices and Cell Culture
Helena Pontes1, Paula Guedes de Pinho1,
Susana Casal2, Helena Carmo1, Agostinho
Santos3, Teresa Magalhães3,
Fernando Remião1, Félix Carvalho1,
and Maria Lourdes Bastos1 1REQUIMTE, Toxicology Department, Faculty of Pharmacy, University
of Porto, Rua Aníbal Cunha 164, 4099-030 Porto, Portugal;
2REQUIMTE, Bromatology Department, Faculty of Pharmacy, University
of Porto, Rua Aníbal Cunha 164, 4099-030 Porto, Portugal;
3National Institute of Legal Medicine I.P., Faculty of Medicine,
University of Porto, Alameda Prof. Hernâni Monteiro, 4200
- 319 Porto, Portugal
A gas chromatography with flame ionization detection
method (GC–FID) with direct injection, using a capillary
column, was validated to determine ethanol, acetaldehyde, methanol,
and acetone in different human matrices, such as whole blood,
vitreous humour, and urine, with clinical and forensic interest.
This method was also employed to quantify these compounds in
cell culture medium, thus being useful in basic research. A good
peak resolution was achieved, with linear correlation between
concentration and peak areas for all the compounds in all the
matrices. The inter- and intra-day precisions of the method were
always under 15% and 10%, respectively. The accuracy of the method,
calculated as the percentage of the target concentration, was
within the acceptable limits. The obtained limits of detection
were below 0.85 mg/L for acetaldehyde and below 0.75 mg/L for
the other considered compounds. The small injection volume and
the high split ratios applied, allied to the high performance
of the GC column, resulted in very good peak resolution and high
sensitivities. This method is easy to perform, making it suitable
for the routine of clinical biochemistry and forensic laboratories.
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