Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 8, September 2009, pp. 728-732
A Simple and Rapid Method for the Determination
of Pennogenin Diglycoside in Rat Plasma by HPLC–MS: Application
to the Pharmacokinetics of the Extract in Gongxuening Capsules
Qingfei Liu1, Yun Shi2, Yuzhen Zhang2 Zhongmin
Song1,
Qiang Su1, Yu Zhang1, and Guoan Luo1
1School of Medicine, Tsinghua University, Beijing, 100084, China
and
2The First Affiliated Hospital, Guangzhou University of Traditional
Chinese Medicine, Guangzhou, 510405, China
A sensitive, selective, and reproducible reversed-phase
high-performance liquid chromatography method coupled with electrospray
ionization-ion trap mass spectrometry (LC–ESI–ITMS)
was developed for the simultaneous quantification of pennogenin
diglycoside (PD) in a small volume (100 µL) of rat plasma.
PD was extracted from rat plasma samples using liquid–liquid
extraction with methanol, digoxin as internal standard. The chromatographic
separation was performed on a reversed-phase Agilent C18 (250
mm × 4.6 mm i.d., 5 μm particle size) analytical column
using a mobile phase of 0.1% formic acid solution–acetonitrile
(75:25, v/v) at a flow-rate of 1.0 mL/min. The mass spectrometer
was operated in the negative ion mode at the deprotonated-molecular
ions [M-H]– of parent drug. Calibration curve in spiked
plasma was linear (correlation coefficient r = 0.999) from 0.5
to 50.0 mg/mL. For the different samples with concentration of
0.50, 5.00, and 50.0 µg/mL, recoveries of PD were (86.45 ± 4.39)%,
(91.40 ± 4.40)%, and (93.79 ± 3.29)%, respectively
(n = 3). The intra-day assay relative standard deviation
at 0.50, 5.00, and 50.0 mg/mL of PD were 4.29%, 5.66%, and 4.03%
(n =
3), respectively. The inter-day assay %RSD at the previously
mentioned concentrations were 5.53%, 4.99%, and 4.31% (n =
3), respectively. The method was successfully applied to the
pharmacokinetic study of PD in rats following either intravenous
administration of PD solution or oral administration of the extract
in Gongxuening capsules, a famous patent Chinese botanic drug.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To
order electronically
click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers. |
Site Map: Home
| Current Issue | Subscribe
| Back Issues | About
Us | Meetings | Advertising
|
| Books for Sale | For
the Author | Links | Supplier
Info | Search | |
|
