Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 8, September 2009, pp. 723-727
Simultaneous Determination of Seven Components
in Qibaomeiran Pill by HPLC–DAD
Chunying Wang, Qiao Wang, Zhifang Yuan, Wei Yang, Fengli
Xie, and Lantong Zhang
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei
Medical University, Shijiazhuang, 050017, P. R. China
A high-performance liquid chromatography coupled
with photo diode array detection method is developed for simultaneous
determination of seven active components in Qibaomeiran pill,
including rutin, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glycoside,
ferulic acid, psoralen, isopsoralen, emodin, and physcion. The
analysis is performed on a C18 column using a mobile
phase composed of A (0.1% acetic acid) and B (acetonitrile) with
linear gradient elution. Four wavelengths at 245, 290, 320, and
350 nm were chosen as the monitoring wavelength to determine
seven active components, respectively. All the compounds show
good linearity (r > 0.999).
The developed method is fully validated in respect to precision,
repeatability, and accuracy. The proposed method is successfully
applied to quantify the seven active components in different
Qibaomeiran pill samples. The results indicate that the developed
assay could be considered as a suitable quality control method
for the Qibaomeiran pill.
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