Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 8, September 2009, pp. 718-722
Study on Determination of Pentacyclic Triterpenoids
in Chaenomeles by HPLC–ELSD
Guangyu Yang1,2,3, Weibo Fen1,2,
Chun Lei1,2, Weilie
Xiao1, and Handong Sun1,
1 State Key Laboratory of Phytochemistry and Plant Resources
in West China, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650204, P.R. China;
2 Graduate School of the Chinese Academy of
Sciences, Beijing 100039, P.R. China;
3 Yunnan Academy of Tobacco Sciences,
Kunming 650106, P.R. China
A new direct high-performance liquid chromatography
analytical method using evaporative light scattering detection
(ELSD) for the simultaneous determination of the major pentacyclic
triterpenoids in Chaenomeles (Chinese medicinal herb)
has been developed. The simultaneous separation of the seven
pentacyclic triterpenoids was achieved on a Zorbax Stable Bound
(4.6 mm × 100
mm i.d., 1.8 µm) C18 column by gradient elution
with acetonitrile–water
as the mobile phase and detected with ELSD. This method provides
good reproducibility and sensitivity for the quantification of
seven major pentacyclic triterpenoids, namely erythodiol, betulin,
acetyl ursolic acid, ursolic acid, oleanolic acid, betulinic
acid, and pomolic acid, respectively. The relative standard deviation
(RSD) of overall intra-day variations was less than 2.2%, and
the RSD of inter-day variations was less than 2.8%. The standard
recoveries (three different concentrations of markers: 0.1, 0.5,
and 2.0 mg) ranged from 96–104%. The results demonstrate
that this method is simple, sensitive, selective, and suitable
for the quality control of this commonly used Chinese medicinal
herb.
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