Published:Journal of Chromatographic Science, ISSN 0021-9665 Volume 47, Number 8, September 2009, pp. 714-717

Simultaneous Determination of Myricitrin, Hyperin, Quercitroside, and Quercetin in Folium Rhododendri Micranthi by RP–HPLC

Xiuling Yang^1,2, Xiaowei Zhang³, Zhifang Yuan¹, Xiaona Li¹, Lantong Zhang¹, and Lifang Fan^1
1 Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, P. R. China;
²The Second Affiliated Hospital of Hebei Medical University, Shijiazhuang, 050000, P.R. China; and
³Department of Metabolic Regulation, Institute of Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621 Japan

A reversed-phase high-performance liquid chromatography method was established for the simultaneous determination of four major constituents, namely myricitrin, hyperin, quercitroside, and quercetin in Folium Rhododendri Micranthi. The optimal conditions of separation and detection were achieved on a C18 analytical column with a gradient mobile phase consisting of acetonitrile and 1% acetic acid at the flow rate of 1.0 mL/min, and detection wavelength was set at 355 nm. All calibration curves showed good linear regression (r > 0.9993) within test ranges. The reproducibility of relative standard deviations was less than 2.3%, and recovery of analytes was greater than 99.4%, respectively. The method was successfully applied to determine the contents of four compounds in Folium Rhododendri Micranthi. The results indicated that the contents of myricitrin, hyperin, quercitroside and quercetin in Folium Rhododendri Micranthi were 0.166%, 0.303%, 0.299%, and 0.053%, respectively.

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