Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 8, September 2009, pp. 709-713
Quantitative Determination of Five Glucosyloxybenzyl
2-Isobutylmalates in the Tubers of Gymnadenia Conopsea and Coeloglossum
Viride var. bracteatum by HPLC
Min Li, Shun-Xing Guo, Chun-Lan Wang, and Pei-Gen Xiao
Institute of Medicinal Plant Development, Chinese Academy of
Medical Sciences and Peking Union Medical College, Beijing 100094, China
Glucosyloxybenzyl 2-isobutylmalates are one group
of important active constituents in the tubers of Gymnadenia
conopsea R. Br. and Coeloglossum viride (L.) Hartm.
var. bracteatum (Willd.). For the purpose
of quality evaluation of these two Chinese herbal medicines,
it is necessary to use a rapid and reliable assay that is suitable
for the determination of their active constituents. A high-performance
liquid chromatography method is firstly developed for the simultaneous
quantification of five glucosyloxybenzyl 2-isobutylmalates in
the tubers. The analytes including dactylorhin B, dactylorhin
E, loroglossin, dactylorhin A, and militarine are isolated from
the tubers of
G. conopsea. The compounds are separated on an Agilent
Hydrosphere C18 (150 × 4.6 mm i.d., 5 µm)
column using a mobile phase of acetonitrile-water including 0.3%
acetic acid (adjusted with 36% acetic acid) with gradient elution
at a flow rate of 1.0 mL/min. Detection is set at a UV wavelength
of 221.5 nm. The recovery of the method is 97.7–101.0%,
and linearity (r > 0.9998) is obtained for all the
analytes. The assay is successfully applied to determine the
contents of the analytes in the tubers of G. conopsea and C.
viride var. bracteatum collected
from different regions of China.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To
order electronically
click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers. |
Site Map: Home
| Current Issue | Subscribe
| Back Issues | About
Us | Meetings | Advertising
|
| Books for Sale | For
the Author | Links | Supplier
Info | Search | |
