Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
47, Number 8, September 2009, pp. 674-680
Characterization of Jamaican Agro-Industrial
Wastes.
Part I: Characterization of Amino Acids Using HPLC: Pre-column
Derivatization with Phenylisothiocyanate
Y.A. Bailey-Shaw1, K.D. Golden1,
A.G.M. Pearson1, and
R.B.R. Porter2
1Department of Basic Medical Sciences (Biochemistry Section)
and
2Department of Chemistry, University of the West Indies, Mona, Kingston
7, Jamaica W.I.
Jamaican agro-industries generate large quantities
of wastes, which are either discarded or under-utilized. In order
to evaluate the possible utilization of these wastes, it is necessary
that the profiles of the major biochemical groups be developed.
This paper describes the determination of the amino acid composition
of coffee, citrus, and rum distillery wastes using a reversed-phase
high-performance liquid chromatography method. Acid hydrolysates
of the wastes are derivatized with phenylisothiocyanate. They
are analyzed as their phenylthiocarbamyl derivatives and determined
quantitatively using norleucine as the internal standard. The
presence of all the 17 amino acids investigated, nine of which
include those essential for animal nutrition, are observed in
the samples investigated, suggesting a high quality of protein
with implications in the formulation of animal feeds.
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