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Published:Journal of Chromatographic Science, ISSN 0021-9665 Volume 47, Number 8, September 2009, pp. 631-635

Flow Injection and Sequential Injection On-line Pre-column Derivatization for Liquid Chromatography

Rodjana Burakham1 and Kate Grudpan2
1Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand and
2Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200 Thailand

Approaches of on-line pre-column derivatization with flow injection (FI) and sequential injection (SI) for liquid chromatography (LC) are reviewed. Considerations in interfacing FI/SI to LC is discussed. Employing such on-line derivatization pre-column pretreatment approaches, applications to organic species (biomedical, food, pharmaceutical, environmental, and other types of samples) as well as metal ions are presented. Further developments in the approaches are recommended.

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