Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 8, September 2008, pp. 743-746
Preparative High-Performance Liquid Chromatography
for the Purification of Natural Acylated Anthocyanins from Red
Radish (Raphanus sativus L.)
Yonghong Liu1, Nobutoshi Murakami2,
Lishu Wang3, and Si Zhang1 1Key Laboratory of Marine Bio-resources Sustainable Utilization,
South China Sea Institute of Oceanology, Chinese Academy of Sciences,
Guangzhou 510-301, China; 2Graduate School of Pharmaceutical
Science, Osaka University, 1-6 Yamada, Suita, Osaka 565-0871,
Japan; 3College of Pharmacy, Changchun University of Chinese
Medicine, Changchun 130117, China
Preparative high-performance liquid chromatography
(HPLC) is applied to the purification of anthocyanins from raw
extracts of red radish (Raphanus sativus L). For each
separation, the chromatographic conditions are optimized to achieve
an efficient purification in the shortest time. In addition,
UV–vis
characterization is carried out on all purified anthocyanins.
The current work shows that analytical chromatographic experiments
alone are useful for the prediction of scale-up conditions of
preparative HPLC separations. Ten known acylated anthocyanins
(See the Appendix for compounds 1–10) are isolated from
the red radish by isocratic HPLC. The structures are established
on the basis of nuclear magnetic resonance and mass spectrometric
analysis. The acylated anthocyanins are all based on pelargonidin
3-sophoroside-5-glucoside, acylated with caffeoyl, feruloyl,
or p-coumaroyl moieties.
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