Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 8, September 2008, pp. 735-738
A Simple High-Performance Liquid Chromatographic
Method for the Estimation of Boswellic Acids from the Market
Formulations Containing Boswellia serrata Extract
Shailesh A. Shah1, Ishwarsinh S. Rathod1,
Bhanubhai N. Suhagia1, Saurabh S. Pandya2,
and Vijay K. Parmar2 1Department of Quality Assurance, L.M. College of Pharmacy, Navrangpura,
Ahmedabad-380 009, India and 2Department of Pharmaceutical Chemistry,
K.B. Institute of Pharmaceutical Education and Research, Sector-23,
Gandhinagar-382023, India
A simple, rapid, and reproducible reverse-phase
high-performance liquid chromatographic method is developed for
the estimation of boswellic acids, the active constituents in
Boswellia serrata oleo-gum resin. The chromatographic
separation is performed using a mobile phase consisting of acetonitrile–water
(90:10, % v/v) adjusted to pH 4 with glacial acetic acid on a
Kromasil 100 C18 analytical column with flow rate of 2.0 mL/min
and detection at 260 nm. The elution times are 4.30 and 7.11
min for 11-keto β-boswellic acid (11-KBA) and 3-acetyl 11-keto β-boswellic
acid (A-11-KBA), respectively. The calibration curve is linear
in the 11.66–58.30 µg/mL and 6.50–32.50 µg/mL
range for 11-KBA and A-11-KBA, respectively. The limits of detection
are 2.33 µg/mL and 1.30 µg/mL for 11-KBA and A-11-KBA,
respectively. The mean recoveries are 98.24% to 104.17% and 94.12%
to 105.92% for 11-KBA and A-11-KBA, respectively. The inter-
and intra-day variation coefficients are less than 5%. The present
method is successfully applied for the estimation of boswellic
acids from the market formulations containing Boswellia serrata
extract.
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