Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 8, September 2008, pp. 730-734
Determination of Tanshinone I in Rat Plasma by
High-Performance Liquid Chromatography and its Application to
Pharmacokinetic Studies
Wei Du1,2, Yu-Hui Wei1,
Gang Zhao1,2, Hong-Yan
Qin1, and Xin-An Wu1, 1Department of Pharmacy, the First Hospital of Lanzhou University,
Lanzhou 730000, PR China; 2College of Pharmacy, Lanzhou University,
Lanzhou 730000, PR China
This paper describes a rapid and sensitive high-performance
liquid chromatographic (HPLC) method for the determination of
the concentration of tanshinone I in rat plasma, and applies
the method to pharmacokinetic study. The plasma is deproteinized
with acetonitrile containing an internal standard (estradiolbenzoate).
The HPLC assay is carried out using a Cosmosil C18 column. The
mobile phase is acetonitrile, 0.05 mol/L–1 ammonium
acetate buffer with 1% acetic acid (66:34, v/v). The flow rate
is 1.0 mL/min. The detection wavelength is set at 263 nm. The
assay accuracy is better than 92%, and the precision of tanshinone
I at low to high concentrations is better than 9% and 11% for
intra-day and inter-day assays, respectively. The recovery of
the method exceeds 88.3% for tanshinone I. The assay shows good
linearity (r = 0.9998) over a relatively wide concentration
range from 0.05 to 10.0 µg/mL. The method is used to determine
the concentration-time profiles of tanshinone I in plasma following
an intravenous injection of tanshinone I solution, and the pharmacokinetic
parameters of tanshinone I are calculated for the first time
by the Drug and Statistics 1.0 program. This assay is successfully
applied to the determination of tanshinone I in rat plasma, and
the developed method is applied to pharmacokinetic studies for
the first time.
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