Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 8, September 2008, pp. 717-721
High-Sensitivity Simultaneous Analysis of Ubiquinol-10
and Ubiquinone-10 in Human Plasma
Aya Hirayama1, Hiroshi Kubo2,
Masashi Mita1,
Osamu Shirota1, and Yorihiro Yamamoto3, 1Shiseido Research Center, 2-2-1 Hayabuchi, Tsuzuki-ku, Yokohama
224-8558, Japan; 2Department of Chemistry and Biotechnology,
Graduate School of Engineering, The University of Tokyo, Hongo,
Bunkyo-ku, Tokyo 113-8656, Japan; 3School of Bionics, Tokyo University
of Technology, 1404-1 Katakura, Hachioji 192-0982, Japan
A method to determine ubiquinol-10 and ubiquinone-10
in human serum was developed by using high-performance liquid
chromatography consisting of a semi-microcolumn switching system
and an electrochemical detector (ECD), which requires minimized
sample pre-treatments. A linear dynamic range was obtained from
1.0 to 5000 ng/mL, and recovery values of 89–105% were
observed in a low-concentration region of 10–50 ng/mL.
In a long operation test, a good precision was maintained during
5100 runs without any maintenance on ECD or columns. In addition,
retention behaviors of other ubiquinone homologues were examined.
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