Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 8, September 2008, pp. 701-706
LC–APCI-MS–MS Method for the Tissue
Distribution of Viaminate After Oral Administrations to Rats
Ling Cao1,2, Feng Zheng1,
Pengcheng Ma3, Wenying
Liu1, Di Sun1, Xuan Chen1, Yanwei
Lai1, and Meng Gou1 1Department of Pharmaceutical Analysis, China Pharmaceutical
University, 210009; 2Jiangsu Institute for Drug Control, 210008,
and 3Institute of Dermatology, CAMS and PUMC, 210042, Nanjing,
China
Fast and sensitive liquid chromatography–atmospheric
pressure chemical ionization-mass spectrometry (LC–APCI-MS)
method for the specific determination of viaminate in 14 kinds
of rat tissues pre-treated with simple procedure was developed
and validated. Biological samples were prepared by direct precipitation
to skin, stomach, intestine, and liver and extracted by liquid–liquid
extraction to lung, kidney, muscle, spleen, brain, fat, testes
(male and female), eye, and heart. After addition of menaquinon
as internal standard to tissue homogenate, the supernatant was
injected into the isocratic chromatographic system using a Waters
Symmetry C8 column and methanol–water–formic acid
(93:7:0.1) as the eluent. The eluate was completely led into
an APCI interface with selected ion monitoring mode and the analytes
were quantified using triple quadrupole MS. The assays were successfully
validated in the ranges 0.02~20 ng/mL for lung, 0.02~10 ng/mL
for kidney, spleen, muscle, brain, fat, eye, and heart, 0.05~10
ng/mL for testicle, 0.4~100 ng/mL for liver, skin and intestine,
and 1.0~200 ng/mL for stomach. The accurate and precise studies
showed good reproducibility with coefficients of variation below
8.5% and the recoveries range from 90 to 109%. The analytes were
chemically stable under all relevant conditions and the assays
were applied in tissue distribution study. The results showed
that the viaminate concentration was high in skin, low in kidney,
and almost undetectable in eye and brain.
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