Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 8, September 2008, pp. 697-700
Determination of Ranolazine in Human Plasma by
Liquid Chromatographic–Tandem Mass Spectrometric Assay
Limei Zhao1, Hao Li2, Yao
Jiang2, Riyang Piao3,
Pengfei Li2, and Jingkai Gu2, 1Second Clinical Hospital affiliated to Chinese Medical University,
Shenyang 110004, P.R. China; 2Research Center for Drug Metabolism,
College of Life Science, Jilin University, Changchun 130021,
P.R. China; 3Jilin Institute of Materia Medica, Changchun 130062,
P.R. China
A highly sensitive liquid chromatographic–tandem
mass spectrometric method (LC–MS–MS) is developed
to quantitate ranolazine in human plasma. The analyte and internal
standard tramadol are extracted from plasma by liquid–liquid
extraction using diethyl ether–dichloromethane (60:40 v/v),
and separated on a Zorbax extend C18 column using
methanol–10mM
ammonium acetate (60:40 v/v, pH 4.0) at a flow of 1.0 mL/min.
Detection is carried out by multiple reaction monitoring on a
QtrapTM LC–MS–MS system with an electrospray ionization
interface. The assay is linear over the range 10–5000 ng/mL
with a limit of quantitation of 10 ng/mL and a lower limit of
detection (S/N > 3) of 1 ng/mL. Intra- and inter-day precision
are < 3.1% and < 2.8%, respectively, and the accuracy is
in the range 96.7–101.6%. The validated method is successfully
used to analyze the drug in samples of human plasma for pharmacokinetic
studies.
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