Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 5, May/June 2008, pp. 450-453
Determination of 5-Fluorouracil in Serum Microsamples
by a High-Performance Liquid Chromatographic Method and its Application
to Pharmacokinetic Studies in Rats
Qiao Wang, Xueying Song, Feng Zhang, and Yongli
Liu
School of Chemical Biology and Pharmaceutical Sciences, Capital
University of Medical Sciences, Beijing 100069, P.R. China
A high-performance liquid chromatographic method
is described for the quantitative determination of 5-fluorouracil
(5FU) in only 30 µL of serum. A reversed-phase C18 column
is used for the separation and analysis of 5FU. The mobile phase
consists of methanol–20mmol/L phosphate buffer, pH 6.80
(5:95, v/v). The calibration curve for 5FU is linear over the
range of 0.05–10 µg/mL. The extraction recoveries
of 5FU and p-aminobenzoic acid (as an internal standard)
are > 91.28%
and 81.98%, respectively. The intra-day and inter-day coefficients
of variation of 5FU are less than 6.29% and 9.57% at four different
concentrations, respectively. The method is simple, sensitive,
and reliable. It is superior to previous methods in that the
sample volume needed is relatively small (just 30 µL of
serum). Therefore, this method can be utilized to determine 5FU
in rats for study in various fields, especially in pharmacokinetics,
controlled-release, and combination therapy with 5FU, etc. In
this study, it is successfully applied to pharmacokinetic studies
of 5FU after administrations of an intravenous bolus dose (25
mg/kg) and two oral doses (25 and 75 mg/kg) in rats.
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