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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 3, March 2008, pp. 254-260
Characteristics of Superficially-Porous Silica
Particles for Fast HPLC: Some Performance Comparisons with Sub-2-µm
Particles
J.J. DeStefano, T.J. Langlois, and J.J. Kirkland
Advanced Materials Technology, Inc., 3521 Silverside Rd., Suite
1-K, Quillen Bldg., Wilmington, DE 19810
Columns of 2.7-µm fused-core (superficially
porous) Type B silica particles allow very fast separations of
small molecules at pressures available in most high-performance
liquid chromatography instruments. These highly-purified particles
with 1.7-µm solid silica cores and 0.5-µm-thick shells
of 9 nm pores exhibit efficiencies that rival those of totally
porous sub-2-µm particles but at one-half to one-third
of the column back pressure. This presentation describes other
operating features of fused-core particle columns, including
sample loading characteristics and packed bed stability. The
superior mass transfer (kinetic) properties of the fused-core
particles result in much-improved separation efficiency at higher
mobile phase velocities, especially for > 600 molecular weight
solutes.
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