Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 3, March 2008, pp. 248-253
Ultrahigh-Pressure Liquid Chromatography: Fundamental
Aspects of Compression and Decompression Heating
R.K. Gilpin and W. Zhou
Brehm Research Laboratory, University Park, Wright State University,
Fairborn, OH 45324-2031
Ultrahigh-pressure liquid chromatography is an
emerging technique for carrying out rapid and highly efficient
separations. Unfortunately, one of the simplifying assumptions
made in conventional high-performance liquid chromatography,
incompressibility of the mobile phase, is not valid when higher
and higher pressures are used. Rather, both compression and decompression
of the eluent must be considered in terms of both heating and
changes in the solvent’s structure. The first of these
problems, eluent heating during the compression and decompression
cycles, which occurs in the pump and column, respectively, are
considered in terms of a combined first principle-empirical approach
that is solved (i.e., an analytic solution obtained from the
resulting integral equation) using 0.01 Bar pressure steps. The
approach is used to estimate compression and decompression heating
for methanol and water.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
