Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 3, March 2008, pp. 220-224
Determination of Paclitaxel in Human Plasma by
UPLC–MS–MS
Shuang-Qing Zhang[1], and Guo-Hua Chen[2]
[1]Department of Pharmaceutics, School of Pharmacy, The University
of Mississippi, MS 38677 USA and
[2]Institute of Vegetables
and Flowers, Chinese Academy of Agricultural Sciences, Beijing
100081, China
A sensitive and specific ultra-performance liquid
chromatography–tandam mass spectrometry method for the
quantitation of paclitaxel is established. A 200 µL human
plasma sample is spiked with 13C6-labeled paclitaxel as an internal
standard and extracted with 1.3 mL of tert-butyl methyl ether.
The chromatographic separation is achieved within 2 min on a
C18 column with methanol–0.1% aqueous formic acid (75:25,
v/v) as a mobile phase at a flow rate of 0.4 mL/min. Multiple
reaction monitoring mass transitions of m/z 876.2 to 307.9 and
882.2 to 313.9 are measured for paclitaxel and the internal standard,
respectively. For paclitaxel at the concentrations of 1.021,
5.105, and 10.21 µg/mL in human plasma, the extraction
recoveries are 105.87%, 103.91%, and 100.39%, respectively. The
linear quantitation range of the method is 0.1021–20.42 µg/mL
in human plasma with a correlation coefficient greater than 0.999.
The intra- and inter-day accuracy for paclitaxel at 1.021, 5.105,
and 10.21 µg/mL levels in human plasma fell in the ranges
of 95.01–99.23% and 95.29–101.28%, and the intra-
and inter-day precision were in the ranges of 6.37–10.88%
and 7.21–9.05%, respectively. This method is successfully
applied to the determination of the half-life of paclitaxel in
human plasma.
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