Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 3, March 2008, pp. 199-208
Pharmaceutical Applications on Columns Packed
with Sub-2 µm Particles
Rossana Russo[2], Davy Guillarme[1], Dao T.-T.
Nguyen[1], Carlo Bicchi[2], Serge Rudaz[1], and Jean-Luc Veuthey[1],
[1]Laboratory of Analytical Pharmaceutical Chemistry, School
of Pharmaceutical Sciences, University of Geneva, University
of Lausanne, Boulevard d’Yvoy 20, 1211 Geneva 4, Switzerland
and
[2]Laboratory of Phytoanalysis, Dipartimento di Scienza e
Tecnologia del Farmaco, Turin University, Via P. Giuria 9-10125
Turin – Italy
During the last few years, there has been a great
interest in the development of fast liquid chromatography. Among
the reported approaches, the reduction of the particle size to
attain sub-2µm diameter represents a good solution for
achieving both increased separation power and faster analysis
time. This paper demonstrates the chromatographic performance
of such supports using plate-height curves and reveals the possibility
for obtaining ultra-fast or highly efficient separations, according
to the column geometry and system pressure limitations. The stability
of these columns is initially evaluated using a system suitability
experiment. The chromatographic performance remains stable in
terms of retention, efficiency, and pressure for more than 1700
injections with pressure conditions ranging from 200 to 800 bar.
Several fast and ultra-fast pharmaceutical applications are reported.
In isocratic mode, a 5- to 10-fold reduction in analysis time
is obtained with limited influence on efficiency and resolution.
The run time is further reduced by 30-fold with the shorter available
columns (i.e., 30 mm length). In gradient mode, the separation
of a complex mixture containing an active pharmaceutical compound
and related impurities is significantly improved with column
length equal to 100 mm, to increase peak capacity and resolution.
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