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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 1, January 2008, pp. 68-73
Combination of Two Hydrophilic Interaction Chromatography
Methods That Facilitates Identification of 2-Aminobenzamide-Labeled
Oligosaccharides
Göran Karlsson, Elin Swerup, and Helena
Sandberg
Octapharma AB, SE-11275 Stockholm, Sweden
Hydrophilic interaction chromatography (HILIC)
is used to separate 2-aminobenzamide- (2-AB) labeled N-linked
oligosaccharides. The glycans of the model protein, bovine fetuin,
are identified following comparison of elution patterns of seven
2-AB-labeled glycan standards, of which two are of the high-mannose
type and five are of the complex type. The combination of two
HILIC methods, using an Amide-80 column, having different resolutions
and selectivities, markedly facilitates the identification of
the fetuin glycans. These HILIC methods are suitable for obtaining
glycan profiles of complex mixtures.
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