Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 1, January 2008, pp. 30-34
Determination of Levodopa and Biogenic Amines
in Urine Samples Using High-Performance Liquid Chromatography
Irena Baranowska and Joanna Plonka
Department of Analytical
and General Chemistry, Silesian University of Technology, 7
M.Strzody Str. 44-100 Gliwice, Poland
A chromatographic system is developed for the
separation and determination of levodopa, biogenic amines, and
their metabolites from the catecholamines group: dopamine (DA),
epinephrine (E), normetanephrine (NMN), metanephrine (MN), 3,4-dihydroxyphenylacetic
acid (DOMA), 3-metoxy-4-hydroxyphenyl-glycol (MHPG), and homovanillic
acid (HVA); and indoloamines group: serotonin (5HT) and 5-hydroxyindole-3-acetic
acid (5HIAA) in urine. The limit of detection (LOD) and limit
of quantitation (LOQ) are determined for all compounds with signal-to-noise
ratio (S/N) of 3 and 10, respectively. LOD 10 (ng/mL) and LOQ
30 (ng/mL) are determined for L-DOPA, DOMA, E, NMN, DA, MN, and
MHPG, as well as LOD 8 (ng/mL) and LOQ 24 (ng/mL) for HVA, 5HT,
and 5HIAA. A fluorescence detector is used. Gradient elution
with acetate buffer (pH = 4.66) with methanol is applied. In
urine samples from patients treated with levodopa, the following
concentrations (µg/mL) of analytes are determined: L-DOPA
3.73–46.80, DOMA 1.43–28.43, E 0.83–13.57,
NMN 2.58–8.81, DA 24.07–62.11, MN 0.89–66.20,
MHPG 6.72–63.64, 5HT 22.96–95.27, 5HIAA 1.45–14.77,
and HVA 0.21–15.07.
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