Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 1, January 2008, pp. 10-14
High-Performance Liquid Chromatography and Thin-Layer
Chromatography for the Simultaneous Quantitation of Rabeprazole
and Mosapride in Pharmaceutical Products
Bhavesh H. Patel[1], Bhanubhai N. Suhagia[2],
Madhabhai M. Patel[1], and Jignesh R. Patel[1]
[1]S.K. Patel College of Pharmaceutical Education and Research,
Ganpat Vidyanagar, Kherva, Mehsana, Gujarat, India and
[2]Department
of Pharmaceutical Chemistry, L.M. College of Pharmacy, Navarangpura,
Ahmedabad, Gujarat, India
Simple, sensitive high-performance liquid chromatography
(HPLC) and thin-layer chromatography (TLC) methods are developed
for the quantitative estimation of rabeprazole and mosapride
in their combined pharmaceutical dosage forms. In HPLC, rabeprazole
and mosapride are chromatographed using 0.01M 6.5 pH ammonium
acetate buffer–methanol–acetonitrile (40:20:40, v/v,
pH 5.70 ± 0.02) as the mobile phase at a flow rate of
1.0 mL/min. In TLC, the mobile phase is ethyl acetate–methanol–benzene
(2:0.5:2.5, v/v). Both the drugs are scanned at 276 nm. The retention
times of rabeprazole and mosapride are found to be 4.93 ± 0.01
and 9.79 ± 0.02, respectively. The Rf values of rabeprazole
and mosapride are found to be 0.42 ± 0.02 and 0.61 ± 0.02,
respectively. The linearities of rabeprazole and mosapride are
in the range of 400–2000 ng/mL and 300–1500 ng/mL,
respectively, for HPLC; in TLC, the linearities of rabeprazole
and mosapride are in the range of 400–1200 ng/spot and
300–900 ng/spot, respectively. The limit of detection is
found to be 97.7 ng/mL for rabeprazole and 97.6 ng/mL for mosapride
in HPLC; in TLC the limit of detection is found to be 132.29
ng/spot for rabeprazole and 98.25 ng/spot for mosapride. The
proposed methods can be applied to the determination of rabeprazole
and mosapride in combined pharmaceutical products.
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