Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 2, February 2008, pp. 184-190
Determination of Danshensu in Rat Plasma and
Tissues by High-Performance Liquid Chromatography
Zi-chuan Zhang, Man Xu, Shi-feng Sun, Hui Guo, Xue Qiao,
Jian Han, Bao-rong Wang, and De-an Guo
The State Key Laboratory of Natural and Biomimetic Drugs, School
of Pharmaceutical Sciences, Peking University, Beijing 100083, People’s
Republic of China
A systematic study on pharmacokinetcs and tissue
distribution of danshensu (one of the major active components
from Salvia miltiorrhiza) is conducted using a rapid and sensitive
high-performance liquid chromatographic (HPLC) method. Before
HPLC analysis, biological samples are pretreated with a liquid–liquid
extraction. Separation of danshensu and internal standard is
achieved on an Agilent Zorbax C18 column with a mobile phase
made up of acetonitrile and 0.05% trifluoracetic acid at a flow
rate of 0.8 mL/min. The calibration curves in plasma and tissues
are linear in the given concentration ranges, with r2 no less
than 0.99. The intra-day and inter-day relative standard deviations
in the measurement of quality control samples are less than 15%,
and the accuracies are in the range of 86–115%. The recoveries
of danshensu in plasma and tissues are among 80% to 118%. Meanwhile,
the multi-peaks in pharmacokinetic profiles are observed. The
method is successfully applied to the pharmacokinetics and tissue
distribution study of danshensu after a single oral administration
of 50.0 mg/kg sodium danshensu to rats.
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