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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 2, February 2008, pp. 169-173
HPLC Determination of Serotonin and Its Metabolites
From Human Platelet-Rich Plasma; Shift to 5-Hydroxytryptophol
Formation Following Alcohol Consumption
Kornélia Tekes
Department of Pharmacodynamics, Semmelweis University, H-1089
Nagyvárad tér Budapest, Hungary
A sensitive, simple, and reliable high-performance
liquid chromatographic method with electrochemical detection
is developed for the measurement of four natural products, the
serotonin-related indols from human platelet-rich plasma (PRP)
using N-methylserotonin as internal standard. Separation of serotonin
(5HT), 5-hydroxytryptophan (5HTP), 5-hydroxytryptophol (5HTOL),
and 5-hydroxyindole-acetic acid (5HIAA) is carried out on Supelcosil
LC-18DB stationary phase. A mixture of 48mM citric acid, 28mM
sodium phosphate dibasic, 0.027mM Na2EDTA, and 3% methanol (pH
3.18) serves as the mobile phase. Measurements are carried out
at 25°C at Eox = 0.65 V. The calibration curves are linear
through the range of 10–200 pg/mL. Method validation is
performed according to internationally accepted criteria. Blood
is collected from healthy controls and schizophrenic subjects.
Significantly higher PRP serotonin is measured in schizophrenics;
patients with recent alcohol consumption could be characterized
with significantly elevated 5HTOL/5HIAA ratio.
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