Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
46, Number 2, February 2008, pp. 162-164
High-performance Liquid Chromatography Analysis
of Four Leuzea carthamoides Flavonoids
Petr Stodulka[1], Vit Koleckar[1,2], Daniel
Junp[1,3], Kamil Kuca[1,3,4], Zuzana Rehakova[2], Katerina
Kubikova[2], Ludek Jahodar[2], Jiri Kunes[5], and Lubomir Opletal[2]
[1]Center of Advanced Studies, Faculty of Military Health Sciences,
University of Defence, Hradec Kralove, Czech Republic;
[2]Department
of Pharmaceutical Botany and Ecology, Faculty of Pharmacy, Charles
University, Hradec Kralove, Czech Republic;
[3]Department of
Toxicology, Faculty of Military Health Sciences, University of
Defence, Hradec Kralove, Czech Republic;
[4]Department of Chemistry,
Faculty of Sciences, J.E. Purkinje University, Usti nad Labem,
Czech Republic; and
[5]Department of Inorganic and Organic Chemistry,
Faculty of Pharmacy, Charles University, Hradec Kralove, Czech
Republic
A simple and sensitive high-performance liquid
chromatography method for the determination of four Leuzea carthamoides
flavonoids, namely eriodictyol, patuletin, eriodictyol-7-b-glucopyranoside,
and 6-hydroxykaempferol-7-O-(6"-O-acetyl-b-D-glucopyranoside),
is presented. Using this method, quantitative composition of
flavonoids ranged from 0.011% to 0.574% in dried plant material
was determined. This method could be used in the future for the
quantitative evaluation of major phenolic compounds in L. carthamoides.
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